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Figures
Fig. 1: Typing results for outbreak-related clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) in the Calgary Health Region (CHR). Left panel: Staphylococcal cassette chromosome mec (SCCmec) typing results. Lanes 1–6, representative outbreak-associated clinical isolates; lane M, molecular size markers, 1 Kb Plus DNA Ladder (Invitrogen). Control strains used: types I (NCTC10442), II (N315), III (85/ 2082), IVa (CA05), IVb (8/6–3P), IVc (MR108), IVd (JCSC 4469) and V (WIS [WBG8318]-JCSC3624), corresponding to distinct amplicon sizes of 613, 398, 280, 776, 493, 200, 881 and 325 bp, respectively. In addition, a 147-bp mecA gene (methicillin-resistant determinant) amplicon was present in all strains. Note: bp = base pairs. Right panel: Pulsed-field gel electrophoresis patterns of USA 300 strains of MRSA circulating in the CHR between Jan. 1 and Sept. 30, 2004. Lane 1, pattern A (31 of 40 cases, 78%); lane 2, USA300 reference strain pattern; lane 3, pattern B (7 cases, 18%); lane 4, pattern C (2 cases, 5%).
Fig. 2: Number of cases that furnished the USA300 strain of methicillin-resistant Staphylococcus aureus (MRSA) in the Calgary Health Region by month of specimen collection, from Jan. 1, 2004 through Sept. 30, 2004 (n = 40; 7 historical isolates were included for comparison). A further 310 infections were identified through ongoing surveillance up to Dec. 31, 2005 (8–34 cases per month).